Current HDAC assay kits have been based on a short wavelength substrate
whose detection is darkened by the presence of autofluorescence interference.
SensoLyte® has ushered in the dawn of ultra-sensitivity.
AnaSpec, EGT Group’s SensoLyte® 520 HDAC Activity Assay Kit is the
industry’s longest wavelength assay kit for HDAC detection (Ex/Em = 490 nm/520
nm). With a longer wavelength, the SensoLyte®520 HDAC Activity Assay
Kit avoids the autofluorescence interference from cell components and test
compounds that is commonly found in traditional HDAC assay kits.
Histone deacetylase (HDAC) enzymes modulate gene expression through the
deacetylation of lysine residues on histone proteins and act as transcriptional
repressors of genes. Based on their role in cell cycling, apoptosis and
differentiation, HDACs have been chosen as therapeutic targets for the
treatment of cancer and neurodegenerative diseases.
520 HDAC Activity Assay Kit provides a convenient, two-step
homogeneous procedure for measuring HDAC activity using a cell-permeable
fluorogenic substrate. Without a time-consuming cell extraction step, this
assay can be used to measure HDAC activity directly in cell culture in a
96-well plate. This kit can also be used for high throughput screening of HDAC
inhibitors using extracts or purified enzymes.
The kit contains: • HDAC 520 substrate • Deacetylated reference standard for
calibration • HeLa nuclear extract • Assay buffer • Cell lysis buffer • HDAC
developer • Trichostatin A (HDAC inhibitor)
AnaSpec also offers the SensoLyte® 440 HDAC Activity Assay Kit as a
cost-effective solution for moderate sensitivity. This kit provides a
convenient, two-step homogeneous procedure for measuring HDAC activity using an
AMC-based fluorogenic substrate (Ex/Em=354 nm/442 nm).